MEDIUM OF CRYOPRESERVATION OF BULL SEMEN AND METHOD OF ITS PREPARATION Russian patent published in 2016 - IPC A01N1/02 A61D19/02 

FIELD: veterinary medicine.

SUBSTANCE: medium for bull semen preservation is composed with use of the following components (wt %): tris-(hydroxymethyl)-aminomethane 1.9, fructose 0.9, citric acid 0.7, sucrose 2, glycerol 4.5-6.5, soybean phospholipids 0.04-2.00, macromolecular surfactant 0.01-1, bidistilled water - the rest. The method of preparing the medium for bull semen cryopreservation comprises preparation of lipid-free base for the medium for bull semen cryopreservation by dilution in hot bidistilled water with a temperature of 80-90°C of tris-(hydroxymethyl)-aminomethane, citric acid, fructose, adding glycerol, and cooling to room temperature, adjusting the solution pH to 6.8. The liposome concentrate is separately prepared by adding in 10% sucrose solution of 0.2-20.0 wt % of soybean phospholipids, the surfactant is applied, it is maintained at room temperature, stirring occasionally for 4-24 hours, then the suspension is treated in the ultrasonic disintegrator for 5-30 minutes and the lipid-free base of the medium is mixed for cryopreservation of bull semen and the liposome concentrate at a ratio of 4:1, sterilised by autoclaving at a temperature of 115°C and pressure of 1.4 of the atmosphere for 1 hour, and sterilized by filtration through a filter with pores of 0.22 mcm.

EFFECT: proposed medium for cryopreservation has improved biological safety, has optical transparency and has a long-term use.

2 cl, 3 tbl, 2 dwg, 4 ex