METHOD OF PROCESSING LIGNOCELLULOSE MATERIAL Russian patent published in 2014 - IPC C12P19/00 C12P7/10 C12N9/14 C12N9/42 C13K1/02 

FIELD: chemistry.

SUBSTANCE: invention relates to field of biochemistry and biotechnology. Performed are: crushing and sieving of lignocelluloses material and selection of granules with particle size from 0.08-0.1 mm. As lignocelluloses material used are: straw, grass, chip, corncobs, bagasse and oil cake. Obtained granules are mixed with weight ratio granules-water 1:(1-5) at temperature 70-90°C. They are dispersed through colloid mill for 1-2 hours to obtain suspension with particle size 40-80 mcm. Homogenisation of obtained suspension is performed under pressure 50-100 atm and temperature 60-85°C for 1-2 hours. Suspension with particles with size 10-40 mcm is obtained. Bufferisation of obtained suspension with buffer solution of sodium acetate and acetic acid with pH value = 4.8-5.8 is carried out. Mixture of enzymes: cellulose in amount 10-60 international units per gram of lignocelluloses material, β-glucosidase in amount 40-100 international units per gram of lignocelluloses material and xylanase in amount 60-120 international units per gram of lignocelluloses material is added. Mixture of enzymes is introduced into reactor after cooling suspension to the temperature of carrying out enzymolysis 40-55°C. Enzymolysis is carried out in reactor for 36-72 hours at rate of reactor rotation 80-160 revolutions per minute. Content of sugar in hydrolysate is determined by method of liquid chromatography.

EFFECT: invention makes it possible to carry out processing of lignocelluloses material at low temperature 40-55°C.

7 cl, 3 dwg, 3 ex