FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology. Method of obtaining complex of organic solvents, which includes acetone, butanol and ethanol, from renewable natural vegetable cellulose-containing raw material includes milling to particles with size 20-80 mcm. Preliminary saccharification of milled raw material is performed for 1-5 h at 45-55°C. The following simultaneous saccharification of carbohydrates and fermentation of obtained hydrolysates are carried out at 28-34°C for 45-50 h under impact of biocatalyst. Said biocatalyst represents cells of bacteria acetobutylicum B-4786, immobilised in cryogel of polyvinyl alcohol in form of cylindrical granules, which have diameter and height 3-5 mm.
EFFECT: method makes it possible to increase productivity of process to 0,464 g/l/h and the total concentration of organic solvents to 23,3 g/l.
4 cl, 9 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PREPARING ORGANIC SOLVENTS | 2009 |
|
RU2405827C2 |
METHOD OF PREPARING ORGANIC SOLVENTS | 2009 |
|
RU2405828C2 |
METHOD OF PREPARING ORGANIC SOLVENTS, APPARATUS FOR REALISING SAID METHOD, PRODUCT OBTAINED USING SAID METHOD | 2008 |
|
RU2405826C2 |
IMMOBILIZED BIOACATALYST FOR BACTERIAL CELLULOSE PRODUCTION | 2016 |
|
RU2636041C2 |
METHOD FOR PRODUCING FUMARIC ACID | 2020 |
|
RU2748229C1 |
BIOCATALYST FOR PRODUCING ETHANOL FROM PENTOSE | 2008 |
|
RU2391402C2 |
METHOD FOR MICROBIOLOGICAL SYNTHESIS OF N-BUTANOL | 2009 |
|
RU2406763C1 |
METHOD OF LACTATE-CONTAINING SOLUTION OBTAINMENT | 2007 |
|
RU2355766C1 |
METHOD OF PREPARING ORGANIC SOLVENTS | 2009 |
|
RU2405829C2 |
METHOD FOR BIOCATALYTIC ANAEROBIC TRANSFORMATION OF EXTRACTS FROM OIL AND OIL FRACTIONS CONTAINING CHEMICALLY OXIDIZED SULPHUR COMPOUNDS | 2021 |
|
RU2803334C2 |
Authors
Dates
2015-01-10—Published
2012-10-31—Filed