IMMUNOLOGICALLY RELEVANT PROTEIN STREPTOCOCCUS Russian patent published in 2014 - IPC G01N33/569 A61K39/09 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics. What is created is an immunologically relevant composition inducing an immune response on at least two strains and/or serotypes Streptococcus uberis, containing at least one recovered and/or recombinant protein having a specific amino acid sequence prepared by the method involving transformation of prokaryotic and eukaryotic cells or a microorganism, such as a bacterium, yeast, or fungi, by a nucleic acid construct coding the above protein, a recombinant nucleic acid molecule, a recombinant carrier and/or their immunologically relevant portion, derivative and/or analogue. A method for preparing the immunologically relevant composition. A method for identifying a protein of the bacterium Streptococcus uberis, that induces the immune response to at least two strains and/or serotypes Streptococcus uberis, involving the stages: a) identifying at least a portion of the secreted protein, superficially associated protein and/or protein min. 80% identical to a sequence of the bacterial virulence factor, b) selecting at least one protein identified at the stage a), which persists in at least two strains and/or serotypes Streptococcus uberis, c) determining an ability of at least one protein selected at the stage b) or its immunologically relevant portion, derivative or analogue to bind specifically the antibody and/or immune cells of an animal infected with the first strain and/or serotype Streptococcus uberis, and the antibody and/or immune cells of an animal infected with the second strain and/or serotype Streptococcus uberis. A method for inducing the immune response on at least two strains and/or serotypes Streptococcus uberis. And also a bovine mastitis diagnostic kit containing at least one protein having the specific amino acid sequence, the recombinant nucleic acid molecule, the recombinant carrier and the agents for detecting the antibodies.

EFFECT: using the invention enables inducing the immune response on at least two strains and/or serotypes Streptococcus uberis.

21 cl, 4 dwg, 7 tbl, 12 ex