METHOD FOR PRECLINICAL TESTING OF IMMUNOMODULATORY AGENTS Russian patent published in 2014 - IPC G01N33/15 A61P37/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, and describes a method for testing a planned or known immunomodulatory agent for T-cell activation which involves the stage of contacting a peripheral blood mononuclear cell (PBMC) culture with the pre-determined amount of the planned or known immunomodulatory agent in vitro and observing the T-cell activation in the PBMC culture using a readout system, when contacting the planned or known immunomodulatory agent, wherein the PBMC culture density at the stage of pre-culture makes at least 2×10⁶/ml, preferentially at least 5×10⁶/ml, more preferentially at least 10⁷/ml, or at least 4×10⁵/cm², preferentially at least 10⁶/cm², most preferentially at least 2×10⁶/cm²; the PBMC pre-culture is cultured for at least 12 hours.

EFFECT: invention provides the improved agent for testing the immunomodulatory agents in vitro.

10 cl, 16 dwg, 13 ex