FIELD: cell biology.
SUBSTANCE: method of producing T-cells and a method for producing a therapeutic agent based on human T-cells, providing for culturing a population of peripheral blood mononuclear cells (PBMC), which contains T-cells and antigen-presenting cells (APC), for 20 hours to 24 hours in a medium containing exogenous interleukin-2 (IL-2), CD3 antibody or CD3-binding fragment thereof and CD28 antibody or its CD28-binding fragment, B7-1 or its CD28-binding fragment, or B7-2 or its CD28-binding fragment; and transducing a PBMC population by a lentiviral vector comprising a polynucleotide encoding a chimeric antigenic receptor (CAR) to a B-cell maturation antigen (BCMA).
EFFECT: this group of inventions can be used in production of adoptive therapy means.
17 cl, 1 tbl, 2 ex, 18 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| IMPROVED COMPOSITIONS BASED ON T-CELLS | 2015 |
|
RU2719030C2 |
| METHOD OF PRODUCTION OF T-CELLS | 2015 |
|
RU2813668C2 |
| CHIMERIC ANTIGENIC RECEPTORS WITH MND-PROMOTER | 2015 |
|
RU2708311C2 |
| METHODS OF EXPANSION OF ANTIGEN-SPECIFIC CAR-T-CELLS, COMPOSITIONS AND THEIR USE | 2019 |
|
RU2800920C2 |
| CHIMERIC ANTIGENIC RECEPTORS WITH MND PROMOTER | 2015 |
|
RU2799573C2 |
| METHODS FOR PRODUCING CELLS EXPRESSING CHIMERIC ANTIGEN RECEPTOR | 2015 |
|
RU2751362C2 |
| METHODS AND COMPOSITIONS FOR OBTAINING GENETICALLY ENGINEERED CELLS | 2018 |
|
RU2795454C2 |
| METHOD AND COMPOSITIONS FOR CELLULAR IMMUNOTHERAPY | 2012 |
|
RU2688185C2 |
| LABELED CHIMERIC EFFECTOR MOLECULES AND RECEPTORS THEREOF | 2014 |
|
RU2729463C2 |
| METHODS OF PRODUCING CELLS EXPRESSING CHIMERIC ANTIGEN RECEPTOR | 2019 |
|
RU2822196C2 |
