FIELD: biotechnology.
SUBSTANCE: method comprises restriction analysis of bacterial DNA of blastocysts using a combination of restriction endonucleases - Hae III, Pst I and Hind III, which enable to identify the protozoa with different degrees of virulence. At that the use of the restriction enzyme Hae III enables to carry out the differentiation of avirulent strains of the blastocysts based on the presence of fragments with size of 850 bps, and the virulent strains based on the presence of DNA fragments with size from 550 to 10000 bps. Using the restriction enzyme Pst I the blastocysts with moderate virulence are revealed based on the presence of DNA fragments with size of about 900 bps. Use of the restriction enzyme Hind III enables to define the type of blastocysts with mild virulence based on DNA fragments with size of 700 bps, and highly virulent strains of blastocysts based on the presence of DNA fragments with size of 380 and 600 bps.
EFFECT: method enables to increase the efficiency of detection of virulence of the protozoa.
6 dwg
