FIELD: biotechnology.
SUBSTANCE: application of yeast strain Komagataella pastoris RNCIM Y-727 as the recipient to construction of producers of target protein is characterised, optionally comprising introduction of mutations into it, providing the use of auxotrophic selective markers.
EFFECT: solution can be applied in preparation of recombinant proteins without the use of methanol as inductor of gene expression.
8 dwg, 4 tbl, 10 ex
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