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2 605 629

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IPC

C12N1/19(2006-01-01)

C12N9/42(2006-01-01)

C12N15/56(2006-01-01)

C12N15/81(2006-01-01)

C12R1/84(2006-01-01)

(21) (22)

Application

2015154051/10, 2015-12-16

(24)

Start date

2015-12-16

(22)

Actual filing date

2015-12-16

(45)

Published

2016-12-27

(72)

Inventor

Berezina Oksana ValentinovnaRykov Sergej ViktorovichZverlov Vladimir VladimirovichSakhibgaraeva Lilija FljuzovnaKrestjanova Irina NikolaevnaJarotskij Sergej Viktorovich

(73)

Holder

Federalnoe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov"

RECOMBINANT STRAIN OF YEAST Pichia pastoris - PRODUCER OF THE SECRETED THERMOSTABLE XYLOGLUCANASE CODED BY SYNTHETIC GENE, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF SECRETED THERMOSTABLE XYLOGLUCANASE BASED ON THIS STRAIN Russian patent published in 2016 - IPC C12N1/19 C12N9/42 C12N15/56 C12N15/81 C12R1/84

Abstract RU 2605629 C1

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, in particular, to strain Pichia pastoris VKPM Y-4269. This is a producer of secreted thermostable xyloglucanase of GH74 class. Said xyloglucanase encoded by synthetic gene corresponding to SEQ ID NO 1. Present invention also relates to a method for microbiological synthesis of xyloglucanase. Above method involves culturing a recombinant microorganisms, containing gene xyloglucanase, in aerobic conditions in a nutrient medium to maximum accumulation of the end product. This nutritional medium includes sources of carbon, nitrogen and mineral additives. Above method is characterised by that is as a producer is used a recombinant strain Pichia pastoris VKPM Y-4269.

EFFECT: present invention enables to obtain secretable thermostable xyloglucanase of class GH74 with high output and purity.

2 cl, 2 dwg, 3 ex

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RU 2 605 629 C1

Authors

Berezina Oksana Valentinovna

Rykov Sergej Viktorovich

Zverlov Vladimir Vladimirovich

Sakhibgaraeva Lilija Fljuzovna

Krestjanova Irina Nikolaevna

Jarotskij Sergej Viktorovich

Dates

2016-12-27—Published

2015-12-16—Filed