METHOD OF PRODUCTION OF FROZEN BACTERIAL CONCENTRATE ON BASIS OF SYMBIOSIS OF PROBIOTIC BACTERIA Russian patent published in 2014 - IPC A23C9/12 C12N1/20 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: method comprises preparing a culture medium, its sterilisation and cooling. In the cooled culture medium the combined inoculum is added, containing separately activated by β-galactosidase bifidobacteria of strain Bifidobacterium longum B 379 M, propionic acid bacteria of strain Propionibakterium Freudenreichii Shermanii subsp. AC-2503, and the activated bacterial inoculum Lactobacillus acidophilus of viscous race taken in a ratio of 9:0.7:0.3, respectively, in an amount of 5% by volume of the culture medium. It is cultured for 14-16 hours at given process parameters, and the cells are separated from the culture liquid to obtain a bacterial mass. The resulting bacterial mass is mixed with the protective medium at a ratio of 1:1. It is bottled, sealed and frozen.

EFFECT: invention enables to intensify the process of fermentation and to improve the structural and mechanical properties of the clot.

2 cl, 7 dwg, 5 tbl, 3 ex