METHOD AND AUTOMATIC DEVICE FOR DIAGNOSTICS OF SEPSIS, CAUSED BY BACTERIA, WHICH CONTAIN GENE NDM-1 Russian patent published in 2014 - IPC G01N33/49 

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, namely to laboratory diagnostics, and can be applied for diagnostics of sepsis, caused by bacteria, which contain a gene NDM-1. For this purpose samples with the bacteria, which contain the gene NDM-1 are subjected to an exposure to ultraviolet probing irradiation, and smears of a patient's blood are analysed. The following analysis of the secondary fluorescence is carried out, with the preliminary multiple measurement of totalities of values of levels of spectral components of the secondary fluorescence from calibrating samples with the bacteria, which contain the gene NDM-1 (pathology), samples of the blood smears, not infected with the bacteria, which contain the gene NDM-1 (norm). After that, totalities of the obtained values of levels of spectral components are determined by pathology and norm as diagnostics criteria, in each analysis of the patient's blood smear samples in a real time mode analogically totalities of the values of levels of spectral components of the secondary fluorescence are multiply measured. The obtained totality of the values of levels of spectral components of the secondary fluorescence as a spectral sample is alternately compared with the preliminarily obtained diagnostics criteria of norm and pathology, the nearest approximation to one of the diagnostics criteria, which determines a diagnosis, is determined. Also claimed is an automatic device.

EFFECT: group of inventions ensures reduction of mortality among the population, the timely beginning of treatment of the said sepsis, resistant to standard antibacterial therapy.

4 cl