DIAGNOSTIC TECHNIQUE FOR AUTOIMMUNE DISORDERS OF GASTROINTESTINAL VEGETATIVE STRUCTURES Russian patent published in 2014 - IPC G01N33/53 G01N33/49 A61B1/00 

FIELD: medicine.

SUBSTANCE: autoimmune disorder of gastrointestinal vegetative structures are diagnosed by examining patient's blood serum. The blood serum sample is examined for neuronal acetylcholine receptor α3-subunit (ACR-α3) and gonadolibarin releasing hormone (GnRH) antibodies by enzyme-linked immunosorbent assay (ELISA) as shown by a reaction to an extracellular portion of neuronal ACR α3-subunit and releasing hormon GnRH, while the ACR-α3 and GnRH levels are determined by straining using tetramethyl benzidine as a chromogene. An optical light absorption is determined by an ELISA analyser at wave length 450 nm in optical density (OD) units. If observing an increase of the ACR-α3 antibodies of more than 0.24 OD, and a decrease of the GnRH antibodies of less than 0.25 OD up to the full absence in relation to a reference, the autoimmune disorder of the peripheral vegetative nervous system at the level of gastrointestinal parasympathetic intramural ganglia and microganglia is diagnosed; if the GnRH antibody level is more than 0.25 OD, and the ACR-α3 antibody level is less than 0.24 up to the full absence, the autoimmune disorder of metasympathetic gastrointestinal intramural ganglia and microganglia is diagnosed.

EFFECT: technique provides high-accuracy determination of the autoimmune involvement of the vegetative nervous structures controlling the gastrointestinal motility, and detecting the involved molecular target, particularly the parasympathetic and/or metasympathetic structures of the gastrointestinal vegetative nervous system.