METHOD FOR TITRATION OF GROUP ABO ANTIBODIES Russian patent published in 2014 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: titration of natural antibodies is ensured by introducing 0.8% test erythrocyte suspension 50 mcl and one of the prepared dilutions of blood serums 25 mcl into each column of a neutral gel card. The prepared mixtures are incubated for 20-25 minutes at 21-23°C. That is followed by centrifugation and titration in limiting dilution to detect visible agglutination in the thickness of the column of the gel card. Titration of complete immune antibodies is ensured by using blood serum 100-200 mcl diluted four times. The serum diluted 1:4 is heated for 10 minutes at temperature 70°. The following dilutions of the heated serum 1:4 are prepared. Further, 0.8% test erythrocyte suspension 50 mcl and one of the prepared dilutions 25 mcl are introduced into each column of the neutral gel card. The prepared mixtures are incubated for 20-25 minutes at temperature 21-23°C, centrifuged and titred. Titration of incomplete immune antibodies is ensured by using blood serum 100-200 mcl diluted four times. The diluted blood serum is heated for 10 minutes at temperature 70°C. The following dilutions of the heated serum 1:4 are prepared. Further, 0.8% test erythrocyte suspension 50 mcl and one of the prepared dilutions 25 mcl are introduced into each column of the gel card with the reagent Coombs. The prepared mixtures are incubated with the gel card or Coombs cards left for 20-25 minutes at temperature 37°C. Thereafter, the gel card or Coombs cards are centrifuged, and the examined antibodies are titred in limiting dilution to detect visible agglutination in the thickness of the column of the gel card.

EFFECT: using of given method enables preventing antibody-mediated rejection reaction in ABO-incompatible transplantations.

2 ex