METHOD OF IDENTIFYING NANODISPERSED SILICON DIOXIDE PARTICLES IN WHOLE BLOOD Russian patent published in 2014 - IPC G01N33/48 B82B1/00 

FIELD: chemistry.

SUBSTANCE: venous whole blood is collected from a worker for whom silicon dioxide is a risk factor in the air of the working area and said blood is divided into two samples. The first sample is analysed by X-ray energy-dispersive microanalysis to determine presence of silicon at the detection threshold level in the sample. The second sample, which is mixed in a volume ratio of 1:1 with heparin solution in concentration of 5000 ME/ml, is analysed by dynamic light-diffusion with photon correlation spectroscopy to determine the size of the detected silicon dioxide particles and construct a first histogram of size distribution of said particles. Dynamic light-diffusion with photon correlation spectroscopy is also used to analyse a first control sample, which is an aqueous suspension of nanodispersed silicon dioxide, while constructing a second histogram of particle size distribution, and a second control sample which is the venous whole blood of an individual not exposed to silicon dioxide mixed with heparin solution with concentration of 5000 ME/ml in volume ratio of 1:1, while constructing a third histogram of particle size distribution. The first, second and third histograms are superimposed and a match between at least part of the peaks on the particle distribution of the first histogram with peaks on the second and third histograms, with simultaneous detection of presence of silicon in the blood sample at the detection threshold level with X-ray energy-dispersive microanalysis, proves presence of nanodispersed silicon dioxide particles in the whole blood.

EFFECT: enabling determination of spatial distribution of nanoparticles in a volume and the behaviour of nanoparticles when analysing diffusion or aggregation and settling processes.

2 cl, 1 tbl, 5 dwg