FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biological engineering, molecular biology, and biotechnology. What is presented is a yeast cell applicable for detecting an interaction between proteins and their domains co-transformed by two plasmids modified for expression of two proteins in the yeast cells, wherein a nucleotide sequence coding the first protein is cloned into one plasmid, while the nucleotide surface coding the second protein - into the other plasmid, wherein the nucleotide sequences coding the proteins are fused with the nucleotide sequences coding the activation and DNA-binding domains of the protein GAL4; the nucleotide sequences coding the studied proteins are separated from the nucleotide sequences coding the activation or DNA-binding domains of the protein GAL4 by means of a nucleotide sequence coding a peptide representing the sequence GluLeuGluAlaAlaAlaLysGluAlaAlaAlaLysGluAlaAlaAlaLysGluAlaAlaAla which is expressed in the form of a protein bridge between the studied protein and the protein GAL4 domains.
EFFECT: invention enables minimising the mutual interaction of the tested protein domains and DNA-binding/activation domains of a vector and can be used for detecting the mutual interaction between the protein molecules for medical and studying applications.
4 dwg, 2 ex
