DOUBLE HYBRID SYSTEM BASED ON GENE SILENCING BY TRANSCRIPTIONAL INTERFERENCE Russian patent published in 2010 - IPC C12N15/65 C12N15/81 G01N33/53 C12Q1/68 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology. The invention describes a yeast cell which contains an interference DNA construct. The construct comprises: a reporter gene under control of a first promoter; one or more in induced promoters called interference promoters selected and fixed in such a way that their activation induces transcriptional interference of the first promoter leading to a detectable decrease in expression of the reporter gene. The cell expresses: a first chimeric protein (Y-AD) formed by a transcription activation domain (AD) fused with a protein Y capable of interacting with at least a partner protein X; a second chimeric protein (X-DBD) formed by a first DNA-binding domain (DBD) fused with a second domain formed by protein X capable of interacting with protein Y, wherein the interaction of the two chimeric proteins X-DBD and Y-AD leads to formation of a functional transcription factor which activates the interference promoter(s). The invention discloses a method of intensifying a compound which inhibits interaction of the first protein X with the second protein Y.

EFFECT: invention enables detection of interruption of protein-protein interaction, as well as identification of alleles lacking on the level of interaction of proteins involved in the protein-protein interactions.

27 cl, 11 dwg, 4 tbl, 11 ex