METHOD FOR ASSESSING ARTIFICIAL LIGHT EXPOSURE ON FUNCTIONS OF PERIPHERAL BLOOD NEUTROPHIL GRANULOCYTES Russian patent published in 2014 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to immunology, and can be used to assess the exposure of artificial light on the innate immune factors. That is ensured by assessing the exposure of light generated by light-emitting diodes, incandescent lamps on the neutrophil granulocytes recovered from a donor's peripheral blood. The neutrophil granulocytes are in vitro exposed to light in the light temperature range of 4000 K with solar radiation of 0.03 W/m² at a wave length of 320 to 400 nm for various periods of time intervals. That is combined with stating the presence or absence of the following variations, such as phagocytic activity increase, lysosomal activity increase, biocidal abilities decrease recorded for the neutropils in the nitro blue tetrazolium reduction test.

EFFECT: using the given method enables assessing the biological safety of the new artificial light sources integrated into an individual's light-colour living environment with using a cell culture of the innate immunity and avoiding direct human participation.

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