BIOPREPARATION FOR CLEANING ENVIRONMENTAL OBJECTS FROM HYDROCARBON POLLUTION, METHOD OF OBTAINING AND APPLICATION THEREOF Russian patent published in 2014 - IPC C12N1/20 C02F3/34 B09C1/10 C12R1/01 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and ecology, namely to protection of the environment. Biopreparation for cleaning environmental objects from petroleum pollution and PAH represents consortium of microorganisms, consisting of the following strains of bacteria: Rhodococcus qingshengii BAC-PAH-1 VKPM AS-1946, Pusillimonas ginsegisoli BAC-PAH-2 VKPM V-11370, Shinella granuli BAC-PAH-3 VKPM V-11371, taken in equal ratios Biopreparation is applied in liquid form of immobilised cell biomass To obtain liquid form submerged cultivation of strains of bacteria, included into consortium, is realised with obtaining cell suspension with its further processing in protective medium To obtain immobilised form of biopreparation submerged cultivation of consortium on nutritional medium is performed with obtaining biomass and further immobilisation of cell biomass on mineral or organic carrier or stabilisation of cell suspension Biopreparation is introduced in amount 0.5-1 l/m² in concentration 1×10⁶-1×10⁷ CFU/g of biopreparation and complex mineral fertiliser - Azophosca, in concentration 20-200 g/m².

EFFECT: invention makes it possible to increase efficiency of cleaning soil from pollution with petroleum, petroleum products and polycyclic aromatic hydrocarbons.

13 cl, 2 tbl, 8 ex