METHOD FOR EVALUATING CONTAMINATION OF PERIODONTAL BY PATHOGENIC BACTERIA USING REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2017 - IPC C12Q1/68 G01N33/48 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, clinical-laboratory diagnostic, in particular to determination of degree of contamination of periodontal pathogenic bacteria by PCR in real-time . Described a method of using complex test system for determining Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis and Treponema denticola, as well as the principle of use of the threshold value, making it possible to distinguish pathological bacterization of parodontium (able to cause the onset of periodontitis) from normal, occurring in the patients without manifested periodontal injury or tendency to it. Method involves PCR in real time for determining in the swabs of periodontal pathogens. Relative value of Ct for sample is determining by the following algorithm: from the averaged in two or more independent determination of absolute value Ct (defined by software thermocycler with an optical detection) for a specific set of primers and probe subtracted average value Ct of total bacterial mass for the same sample series, then identified pathological periodontal bacterization provided that value of relative Ct does not exceed 15.

EFFECT: invention can be used for detection of causes of chronic periodontitis in individual patient, evaluating risk of occurrence of this disease in the future and clinical effectiveness as aggressive and chronic periodontitis.

1 cl, 6 dwg