FIELD: biotechnologies.
SUBSTANCE: method is implemented using a medium containing no proteins and serum, containing an ultrafiltrated hydrolysate of soy in a concentration of 0.5-50 g/l, where at least 40% of the above soy hydrolysate has a molecular weight of 200-500 Dalton. The above medium also contains an amino acid chosen from a group consisting of L-asparagine, L-cysteine, L-cystine, L-proline, L-triptophane, L-glutamine or their mixtures. The invention describes use of the above medium for cultivation of recombinant cells of mammals, a cultivation method of cells in such medium, a composition containing such medium, as well as its production.
EFFECT: use of above medium allows improving production of factor VIII with recombinant cells with simultaneous elimination of shortages of cultivation with no serum.
11 cl, 4 dwg, 7 tbl, 9 ex
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|
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| CELL CULTIVATION MEDIUM WITHOUT PROTEINS AND SERUM | 2000 |
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| MEDIUM FOR CELLS CULTIVATION THAT DOES NOT CONTAIN ANIMAL PROTEINS | 2005 |
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| METHOD OF PROTEIN EXPRESSION | 2007 |
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| MEDIUM FOR CELL CULTURE THAT DOES NOT CONTAIN OLIGOPEPTIDES | 2017 |
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| OLIGOPEPTIDE-FREE CELL CULTURE MEDIA | 2013 |
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|
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| METHOD FOR PREPARING AND ISOLATING PROTEIN ELICITING ACTIVITY OF FACTOR VIII, STRAIN OF CELLS HKB EXPRESSING PROTEIN ELICITING ACTIVITY OF FACTOR VIII (VARIANTS) | 1999 |
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| RECOMBINANT PLASMID DNA pAP227 CODING POLYPEPTIDE OF RECOMBINANT FACTOR VIII OF HUMAN BLOOD COAGULABILITY, LINE OF CELLS Cricetulus griseus CHO, 2H5-PRODUCER OF RECOMBINANT FACTOR VIII OF HUMAN BLOOD COAGULABILITY, AND METHOD FOR OBTAINING POLYPEPTIDE HAVING ACTIVITY OF RECOMBINANT FACTOR VIII | 2012 |
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RU2500818C1 |
| METHOD FOR PREPARING FED-BATCH ANTIBODY OR ITS FRAGMENT (VERSIONS) | 2007 |
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RU2518289C2 |
