FIELD: biotechnology.
SUBSTANCE: method of protein expression in a medium comprising at least 0.5 mg/l of polyamine is proposed. The method provides obtaining a cell culture, the insertion into cells of nucleic acid encoding the target protein, the selection of transformed cells and culturing the cells in a medium containing at least 0.5 mg/l of polyamine and free of oligopeptides.
EFFECT: improvement of the method.
10 cl, 7 dwg, 11 ex
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Authors
Dates
2013-06-27—Published
2007-01-03—Filed