FIELD: biotechnology.
SUBSTANCE: method for increasing the expression of a recombinant protein in a mammalian cell culture is proposed, including cultivating cells expressing a recombinant protein in protein-free medium that does not contain oligopeptides supplemented with at least 2 mg/l of putrescine 2HCl and containing amino acids, vitamins, organic and inorganic salts and carbohydrate sources.
EFFECT: invention provides for an increase in the expression of a recombinant protein due to an increase in the growth and specific productivity of mammalian cells for the production of biological products used in pharmaceutical preparations.
19 cl, 7 dwg, 11 ex
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