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Application

99101688/13, 1999-02-03

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Start date

1999-02-03

(22)

Actual filing date

1999-02-03

(45)

Published

2000-09-20

(72)

Inventor

Markin S.S.(Ru)Spizhenko Jurij Prokof'EvichMarkov A.P.(Ru)Nechaeva E.V.(Ru)Ljashenko A.A.(Ru)Uvarov V.Ju.(Ru)Tarasov Aleksandr AndreevichZhukova A.V.(Ru)Semenov M.P.(Ru)

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Holder

Zakrytoe Aktsionernoe Obshchestvo

RECOMBINANT PLASMID DNA ENCODING COMPLETE SEQUENCE OF STAPHYLOKINASE, STRAIN OF ESCHERICHIA COLI SA 9325 AS PRODUCER OF RECOMBINANT PROTEIN Russian patent published in 2000 - IPC

Abstract RU 2156299 C1

FIELD: molecular biology, microbiological and medicinal industries, pharmacy. SUBSTANCE: invention proposes recombinant DNA containing the complete sequence of staphylokinase and lider block consisting of 6 histidine residues. Strain of E. coli SA 9325 is a producer of recombinant protein containing the complete sequence of staphylokinase from S. aureus. Invention ensures to obtain highly active protein showing fibrinolytic activity. The yield of recombinant protein is 20-26% of total protein content. Invention can be used for development of drugs, in part, recombinant forms of staphylokinase. EFFECT: recombinant DNA indicated above, high yield and activity of recombinant protein. 3 cl, 5 dwg, 1 ex

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RU 2 156 299 C1

Authors

Markin S.S.(Ru)

Spizhenko Jurij Prokof'Evich

Markov A.P.(Ru)

Nechaeva E.V.(Ru)

Ljashenko A.A.(Ru)

Uvarov V.Ju.(Ru)

Tarasov Aleksandr Andreevich

Zhukova A.V.(Ru)

Semenov M.P.(Ru)

Dates

2000-09-20—Published

1999-02-03—Filed