FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, particularly to genetic engineering, can be used to identify a genetic material (RNA) of human respiratory syncytial virus in samples, and can be used in microbiology and medicine. A set of oligodeoxyribonucleotide primers for detecting RNA of human respiratory syncytial virus and comprises two pairs of oligonucleotide polymers possessing an activity of upstream and downstream primers in polymerase chain reaction, as well as two fluorescence marked DNA probes having the follow structure: Outer upstream primer (nested forward primer) 5'→3' F: 5'-AGRCARCARAGTTAYTCYATYATGTC-3' (26 s.); outer downstream primer (nested reverse primer) 5'→3' R: 5'-AATTTATTATWGGTTYMCCYTTTACATA-3' (25 s.); upstream primer (forward primer) 5'→3' F: 5'-GACACHATGAAYAGYTTRACATTACC-3' (26 s.); downstream primer (reverse primer) 5'→3' R: 5'-AAATGTYTTTATDATYCCRCGATTT-3' (25 s.); fluorescence marked DNA probes (probe) 5'→3' Z: 5'-FAM-TCTCTAGGAGCCATTGTGTCATGCTATGG-BHQ1-3' (29 s.) Z: 5'-FAM-TCTCTWGGAGCYATAGTGTCATGYTATGG-BHQ1-3' (29 s.).
EFFECT: extended spectrum of import-substituting oligodeoxyribonucleotide primers and fluorescence marked probes enabling identifying RNA of human respiratory syncytial virus by real-time PCR.
1 cl, 5 tbl, 1 dwg
| Title |
Year |
Author |
Number |
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2015 |
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2013 |
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2011 |
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2020 |
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2011 |
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