FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering, and can be used to detect genetic material (RNA) of human coronaviruses SARS and 2019-nCoV in clinical samples. Disclosed is a set of oligodeoxyribonucletide primers and a fluorescently labelled DNA probe for identifying human coronaviruses SARS and 2019-nCoV by RT-PCR with hybridisation-fluorescent detection. Kit contains one pair of oligonucleotides having activity of forward and reverse primers in a polymerase chain reaction, as well as a fluorescent-labelled DNA probe. Straight (F4) and reverse (R5) primers have sequences: F4: 5'-GTTGCAACTGAGGGAGCCTTG-3', R5: 5'-GAGAAGAGGCTTGACTGCCG-3' respectively. Fluorescent-labelled DNA probe Pb1: 5'-FAM-TACACCAAAAGATCACATTGGCACCCG-BHQ1-3'. Invention provides a sensitive and specific kit for detecting human coronaviruses SARS and 2019-nCoV by PCR with hybridisation-fluorescent detection in real time in clinical samples.
EFFECT: presented kit detects genetic material of coronavirus SARS in concentration 1 × 103 copies/ml in 85_7 % of cases, sensitivity is not less than 50 genome equivalents per reaction.
1 cl, 5 dwg, 4 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFICATION OF HUMAN CORONAVIRUS 2019-nCoV RNA BY PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2020 |
|
RU2734300C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES FOR THE IDENTIFICATION OF HUMAN CORONAVIRUS SARS-COV-2 RNA BY ISOTHERMAL PCR WITH REAL-TIME HYBRIDIZATION-FLUORESCENCE DETECTION | 2021 |
|
RU2778855C1 |
| TEST SYSTEM FOR DETECTING SARS-CoV-2, INFLUENZA VIRUS A, INFLUENZA VIRUS B BY THE METHOD OF ONE-STEP POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION | 2021 |
|
RU2761481C1 |
| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
|
RU2752902C1 |
| SET OF OLIGODESOXYRIBONYCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBE FOR IDENTIFICATION OF RNA OF HUMAN CORONAVIRUS ASSOCIATED WITH SEVERE SHARP RESPIRATORY SYNDROME | 2012 |
|
RU2504585C1 |
| SET FOR IDENTIFYING SARS-CoV-2 CORONAVIRUS | 2021 |
|
RU2765497C1 |
| SET OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES AND A METHOD FOR QUANTIFYING THE VIRAL LOAD OF SARS-CoV-2 IN TISSUES OF VARIOUS ORGANS BY QUANTITATIVE POLYMERASE CHAIN REACTION IN REAL TIME | 2021 |
|
RU2761358C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF SARS-CoV-2 MUTATION S:L452R | 2022 |
|
RU2795018C1 |
