FIELD: biotechnology.
SUBSTANCE: invention is a method of producing an adjuvant for vaccines, comprising dissolving a mixture of triterpenoids of bark in tetrahydrofuran, addition of oleic acid, removal of tetrahydrofuran, addition of cryoprotectant, and lyophilisation, at that a mixture of triterpenoids of bark in tetrahydrofuran at a concentration of 5-10 g/l is obtained, followed by dissolving the resulting mixture in oleic acid in an amount of 5-10% by weight of triterpenoids of bark, sterilising filtration of the mixture is carried out, a homogeneous dispersion of spherical amorphous nanoparticles is formed by adding a 25-fold excess of 0.01 M tris buffer, pH - 9.0±0.2 with stirring, followed by ultrasonic treatment for 5-10 minutes, tetrogidrofuran is removed by ultrafiltration at a rate of 1.0-1.2 l/min at a pressure of 0.6-0.8 atm, adding the cryoprotectant the resulting concentrated mixture containing a mixture of terpenoids of 1 mg/ml with the temperature below minus 35°C is frozen, kept at this temperature for 4-6 hours, and lyophilised at a temperature of minus 35°C for 15 hours, followed by subsequent drying at 20-25°C for 15 hours.
EFFECT: invention enables to increase the immunogenic activity of viral vaccines and provides their stability while storage.
3 cl, 2 dwg, 6 tbl, 7 ex
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Authors
Dates
2015-04-10—Published
2014-02-19—Filed