METHOD OF PRODUCTION OF ADJUVANT FOR VIRAL VACCINES Russian patent published in 2015 - IPC A61K36/185 

FIELD: biotechnology.

SUBSTANCE: method comprises dissolving the mixture of birch bark triterpenoids in tetrahydrofuran to obtain the solution with a concentration of 5-10 g/l. Oleic acid is dissolved in an amount of 5-10% by weight of the birch bark triterpenoids. The sterilising filtration of the mixture is carried out. 25-fold excess of 0.01 M tris buffer is added, pH 9.0±0.2, when stirring. Sonication is carried out for 5-10 min. The organic solvent is removed using ultrafiltration on hollow membranes with exclusion threshold of 300 kDa at a rate of 1.0-1.2 L/min at a pressure of 0.6-0.8 atm. Cryoprotectant is added from the group of substances: mannitol, maltose, trehalose, mannose, sorbite, sucrose. The resulting concentrated mixture is frozen.

EFFECT: invention enhances the immunogenic activity of viral vaccines and provides their stability while storage.

2 cl, 2 dwg, 6 tbl, 7 ex