FIELD: biotechnology.
SUBSTANCE: group of inventions is the recombinant strain Escherichia coli RNCIM B-11864, which has constitutive aspartase activity and obtained by introducing into the E. coli strain RNCIM B 7188 of plasmid containing aspA gene under control of the promoter pEftu from Corynebacterium glutamicum ATCC13032. The group of inventions also relates to a method of synthesis of L-aspartic acid, according to which ammonium fumarate is mixed with a suspension of the biocatalyst, and the biocatalyst is used as the said recombinant strain.
EFFECT: group of inventions enables to obtain L-aspartic acid with high efficiency.
3 cl, 1 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PLASMID-FREE RECOMBINANT ESCHERICHIA COLI STRAIN, WITH CONSTITUTIVE ASPARTASE ACTIVITY AND METHOD FOR L-ASPARAGIN ACID SYNTHESIS USING THIS STRAIN AS BIOCATALYST | 2015 |
|
RU2620942C2 |
| METHOD OF L-ASPARTIC ACID PRODUCING | 2000 |
|
RU2174558C1 |
| RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2499830C1 |
| STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2502797C1 |
| BACTERIA OF ESCHERICHIA KIND - PRODUCER OF L-THREONINE AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF L-THREONINE WITH ITS USAGE | 2012 |
|
RU2515095C1 |
| RECOMBINANT BACTERIUM STRAIN Rhodococcus rhodochrous HAVING CONSTITUTIVE ACYLATING ACTIVITY, AND METHOD OF SYNTHESIS OF N-REPLACED ACRYLAMIDES USING THIS STRAIN AS BIOCATALYST | 2013 |
|
RU2539033C1 |
| RECOMBINANT PLASMID DNA PSA V27 ENCODING SYNTHESIS OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII AND BACTERIAL STRAIN ESCHERICHIA COLI AS PRODUCER OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII | 1999 |
|
RU2153535C1 |
| BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF RECOMBINANT EXTRACELLULAR DOMAIN OF PROTEIN GP | 2018 |
|
RU2701337C1 |
| METHOD FOR PREPARING L-THREONINE, STRAIN ESCHERICHIA COLI AS PRODUCER OF THREONINE (VARIANTS) | 2002 |
|
RU2244007C2 |
| 85 ESCHERICHIA COLI STRAIN AS PRODUCER OF L-ASPARTIC ACID | 0 |
|
SU644833A1 |
