FIELD: biotechnology.
SUBSTANCE: plasmid-free recombinant Escherichia coli strain with constitutive aspartic activity and obtained by replacing the promoter of the aspA gene in Escherichia coli strain MG1655 with the bacteriophage T5 pG25 promoter is represented. A method for L-aspartic acid synthesis from ammonium fumarate is provided, using the said recombinant strain as a biocatalyst in the form of either free or immobilized cells.
EFFECT: more efficient preparation of L-aspartic acid; and allows to reduce the time of its production 3-4 times due to higher aspartic activity of the biocatalyst.
2 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN Escherichia coli, HAVING CONSTITUTIVE ASPARTASE ACTIVITY AND METHOD OF SYNTHESIS OF L-ASPARTIC ACID USING THIS STRAIN AS BIOCATALYST | 2013 |
|
RU2546239C1 |
| METHOD OF L-ASPARTIC ACID PRODUCING | 2000 |
|
RU2174558C1 |
| 85 ESCHERICHIA COLI STRAIN AS PRODUCER OF L-ASPARTIC ACID | 0 |
|
SU644833A1 |
| BACTERIA OF ESCHERICHIA KIND - PRODUCER OF L-THREONINE AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF L-THREONINE WITH ITS USAGE | 2012 |
|
RU2515095C1 |
| METHOD OF OBTAINING L-ASPARTIC ACID | 0 |
|
SU659611A1 |
| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
|
RU2422524C1 |
| RECOMBINANT BACTERIAL STRAIN Escherichia coli THAT IS SUCCINIC ACID PRODUCER (VERSIONS) AND METHOD FOR PREPARING SUCCINIC ACID WITH USING THIS STRAIN | 2012 |
|
RU2528056C2 |
| MUTANT ADENYLATE CYCLASE, DNA CODING IT, BACTERIA OF ENTEROBACTERIACEAE FAMILY CONTAINING SAID DAN AND METHOD FOR PREPARING L-AMINO ACIDS | 2010 |
|
RU2471868C2 |
| METHOD OF PRODUCTION OF L-AMINO ACIDS USING BACTERIA OF FAMILY Enterobacteriaceae | 2010 |
|
RU2497943C2 |
| AGENT PRODUCING RESISTANCE OF CELLS TO PHAGOLYSIS | 0 |
|
SU1463759A1 |
