RECOMBINANT BACTERIUM STRAIN Rhodococcus rhodochrous HAVING CONSTITUTIVE ACYLATING ACTIVITY, AND METHOD OF SYNTHESIS OF N-REPLACED ACRYLAMIDES USING THIS STRAIN AS BIOCATALYST Russian patent published in 2015 - IPC C12N1/20 

Abstract RU 2539033 C1

FIELD: biotechnologies.

SUBSTANCE: invention relates to recombinant bacterium strain Rhodococcus rhodochrous RNCIM Ac1960, which has a constitutive acylating activity and is obtained by replacement in a chromosome of strain Rhodococcus rhodochrous RCM Ac-1515D of a gene coding nitril hydrase with a gene coding acylamidase from strain Rhodococcus erythropolis 37 RNCIM Ac-1793, as well as to a synthesis method of N-replaced acrylamides from acrylamide and amines using it as a biocatalyst.

EFFECT: invention allows efficient production of concentrated solutions of N-replaced acrylamides.

2 cl, 1 dwg, 1 tbl, 5 ex

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RU 2 539 033 C1

Authors

Lavrov Konstantin Valer'Evich

Novikov Andrej Dmitrievich

Rjabchenko Ljudmila Evgen'Evna

Gerasimova Tat'Jana Vasil'Evna

Janenko Aleksandr Stepanovich

Dates

2015-01-10Published

2013-10-21Filed