FIELD: biotechnology.
SUBSTANCE: method of preparing the nutrient medium for cultivation of lactobacilli comprises preliminary preparing of acid hydrolysate of blood of slaughtered animals, yeast autolysate and milk whey with known methods. The acid hydrolyzate of blood of slaughtered animals is deoxidised to pH of 6.7-6.9 and is added to yeast autolysate, milk whey, glucose and cabbage broth in a predetermined ratio of components. Then the mixture is heated and boiled for 5-7 minutes. The pH of the culture medium is determined and adjusted to 6.7-6.9. The resulting nutrient medium is filtered and packaged in vials, followed by sterilisation by autoclaving at 0.5 atm for 25-30 minutes.
EFFECT: invention enables to increase the yield of biomass and to increase the intensity of its growth.
3 tbl, 3 ex
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Authors
Dates
2015-05-10—Published
2014-04-04—Filed