METHOD OF IMMOBILISATION OF CHYMOTRYPSIN ON NANOPARTICLES OF SELENIUM OR SILVER Russian patent published in 2015 - IPC C12N11/14 C12N9/76 B82B3/00 C01G5/00 C01B19/00 

FIELD: biotechnology.

SUBSTANCE: method of immobilisation of chymotrypsin on nanoparticles of selenium or silver is proposed. The solution of chymotrypsin at a concentration of from 1·10^-3 to 1 wt % is added the solution of selenious acid in the concentration range of 1.3·10^-4-1.5 wt % or silver nitrate in the concentration range of 1·10^-4-1 wt %. Then ascorbic acid is added to the reaction medium in the concentration of 1·10^-3-0.7 wt % or solution of sodium borohydride in a concentration of from 0.01 to 0.6 wt %. The solution is stirred and left to complete the reaction. The process is carried out at a temperature of from 0 to 50°C.

EFFECT: method enables to obtain a stable enzyme-colloidal complex and maintain in it over 90 percent of chymotrypsin activity in a broad pH range, and to increase the catalytic activity of nanocomplexes in certain pH ranges higher than the activity of native chymotrypsin at optimum point.

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