FIELD: biotechnology.
SUBSTANCE: animal is placed on the movable thermostated preparation microscope stage. Median laparotomy is carried out. Bleeding is stopped by thermocoagulation, fixing the wound edges using ligatures. Paramedial incision is made in the right at the level of lower third of abdomen, through which the removable hollow light guide of cylindrical shape is inserted into the abdominal cavity and it is filled with indifferent oils or normal saline. The mesentery of small intestine is thrown on the upper end of the light guide and biomicroscopy is carried out using a fixed camera for a microscope, connected to the personal computer, the results are recorded at various stages of research, followed by treatment of the data obtained, using a computer program.
EFFECT: method eliminates drying and adhesion of the area under study, as well as bending the vessels, which enables to perform long-term studies.
3 dwg
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Authors
Dates
2015-07-10—Published
2014-04-23—Filed