FIELD: medicine.
SUBSTANCE: invention relates to field of biotechnology and deals with method of separating mixture of DNA and proteins from epimastigote forms of strain TPAP/MX/2002/Albarrada of Trypanosoma cruzi culture. Characterised method includes obtaining biomass of said strain culture, with further washing of obtained biomass, its concentration to content of not less than 6×108 cells in 1 ml, introduction of synthetic preparation of human defensin α-1 with molecular weight 3.4 kDa into obtained concentrated biomass in amount 20 mcg/ml to its final concentration 3.97 mcM and exposure until pores are formed in lipid membranes. After that obtained suspension is shaken by shaker with further exposure at room temperature for not less than 24 hours, with the following centrifuging obtained medium with separation of mixture of DNA and proteins in liquid phase.
EFFECT: invention makes it possible to obtain product with reduced content of protein components and can be used in further obtaining of preparations.
2 cl, 2 ex
