METHOD OF PREPARING HETEROLOGOUS PROTEINS Russian patent published in 2013 - IPC C12P21/00 C12N1/15 C12N5/10 C12N9/10 

Abstract RU 2487168 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of preparing a pharmaceutical composition, CHO cell to obtain the desired protein, the CHO cell - DNA recipient encoding the desired polypeptide, the method of production of the desired polypeptide. The method of production of the desired polypeptide comprises cultivation of CHO cell which is transformed DNA encoding alanine aminotransferase and DNA encoding the desired polypeptide. In the particular case the CHO cell is cultured in α-ketoglutarat-containing medium. The method of preparing of the pharmaceutical composition comprises preparing of the desired polypeptide with the method described above. The polypeptide is mixed with pharmaceutically acceptable carriers or additives. The preparation is prepared. The CHO cell for preparing of the desired protein has DNA transferred into it, encoding alanine aminotransferase, and DNA transferred into it, encoding the desired polypeptide.

EFFECT: invention enables to prepare a desired polypeptide with a high yield.

10 cl, 22 dwg, 3 ex

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RU 2 487 168 C2

Authors

Tabuti Khisakhiro

Sugijama Tomoja

Dates

2013-07-10Published

2008-08-06Filed