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2 827 613

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IPC

C12N15/23(2006-01-01)

C12N15/70(2006-01-01)

C12N1/21(2006-01-01)

C12P21/02(2006-01-01)

(21) (22)

Application

2023109060, 2023-04-10

(24)

Start date

2023-04-10

(22)

Actual filing date

2023-04-10

(45)

Published

2024-09-30

(72)

Inventor

Shcherbakov Dmitrij NikolaevichVolosnikova Ekaterina AleksandrovnaVolkova Natalya VyacheslavovnaEsina Tatyana IgorevnaTereshchenko Tamara AnatolevnaGogina Yana Stanislavovna

(73)

Holder

Federalnoe Byudzhetnoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka Gnts Vb Rospotrebnadzora)

RECOMBINANT PLASMID DNA pET21-IFN- γ, CONTAINING GENE CODING HUMAN GAMMA-INTERFERON, ESCHERICHIA COLI BL21/pET21-IFN-γ STRAIN - PRODUCER OF HUMAN RECOMBINANT GAMMA-INTERFERON AND METHOD OF PRODUCING HUMAN GAMMA-INTERFERON Russian patent published in 2024 - IPC C12N15/23 C12N15/70 C12N1/21 C12P21/02

Abstract RU 2827613 C1

FIELD: biotechnology.

SUBSTANCE: disclosed is recombinant plasmid DNA pET21-IFN-γ providing synthesis of a polypeptide having human gamma-interferon activity. Also disclosed is a recombinant bacterium Escherichia coli, which is a producer of a polypeptide having human gamma-interferon activity and containing said recombinant plasmid DNA. Also disclosed is a method of producing recombinant human gamma-interferon using said bacteria.

EFFECT: invention provides a wider range of products for producing recombinant human gamma-interferon protein, as well as higher expression level of said protein.

3 cl, 5 dwg, 2 tbl, 8 ex

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RU 2 827 613 C1

Authors

Shcherbakov Dmitrij Nikolaevich

Volosnikova Ekaterina Aleksandrovna

Volkova Natalya Vyacheslavovna

Esina Tatyana Igorevna

Tereshchenko Tamara Anatolevna

Gogina Yana Stanislavovna

Dates

2024-09-30—Published

2023-04-10—Filed