RECOMBINANT PLASMID DNA pGST/ART/X, CODING FUSED CHIMERIC POLYPEPTIDE GST/ART/X FOR HIGH SPECIFIC SELECTION OF APTAMERS TO TARGET PROTEIN X IN POLYPEPTIDE COMPOSITION Russian patent published in 2015 - IPC C12N15/66 C12N15/62 C12N15/70 

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biotechnology and can be applied for the selection of affine molecules. Constructed is a plasmid DNA pGST/APT/X 6193 bp long, with the weight of 4.09 MDa, containing as a genetic marker the gene β-lactase and unique sites of the recognition of restriction endonucleases, located at the following distance to the right from the site NdeI: BgIII 665bp, BamHI 779 bp., XhoI 801 bp. The plasmid pGST/APT/X consists of a NdeI-XhoI DNA fragment of the commercial plasmid pET22b(+) and nucleotide sequence SEQ ID NO:1, inserted by sites NdeI-XhoI and combining in its composition the sequences, which code a fragment of a gene of glutathione-S-transferase, a peptide substrate of a lethal factor of anthrax RRKKVYPYPME, peptide GGLNDIFEAQKIEWHED, biotinylated in vivo under the influence of E.coli biotin-ligase, and a linker sequence, substituted by genetic-engineering manipulations by sites of restriction endonucleases BamHI and XhoI for a sequence, coding a target protein B-subunit of shiga-toxin I.

EFFECT: invention makes it possible to carry out the selection of highly specific aptamers to the said target protein.

2 cl, 4 dwg, 4 ex