RECOMBINANT PLASMID DNA PNDCTR1 CODING HYBRID POLYPEPTIDE GST-NDCTR1, ESCHERICHIA COLI BL21 (DE3)/PNDCTR1 BACTERIA STRAIN - PRODUCER OF HYBRID POLYPEPTIDE GST-NDCTR1 AND HYBRID POLYPEPTIDE GST-NDCTR1, MAKING CHELATES OF COPPER, SILVER AND PLATINUM IONS Russian patent published in 2016 - IPC C07K19/00 C07K14/47 C12N15/62 C12N15/70 C12N1/21 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used for recombinant production of CTR1. Method comprises producing plasmid DNA pNdCTR1 coding hybrid polypeptide GST-NdCTR1, consisting of the N-terminal polypeptide fragment of glutathione-S-transferase (GST), connected via thrombin hydrolysis site with the polypeptide fragment of the N-terminal domain of a high-affinity human copper CTR1 (NdCTR1) importer. Size of the plasmid DNA is 5149 bps, the plasmid DNA comprises BamHI-XhoI - pGEX-4T-1 fragment with size of 4951 bps and SLC31A1 gene fragment with size of 198 bps, encoding the N-terminal domain of human CTR1 protein. Nucleotide NdCTR1 sequence and GST sequence are in the same reading frame. E.coli BL21(DE3) cells are transformed with the produced DNA pNdCTR1 to obtain an E.coli BL21 (DE3)/pNdCTR1 producer strain.

EFFECT: invention allows producing hybrid polypeptide GST-NdCTR1 making chelates of copper, silver and platinum ions.

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