FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and represents a DNA-aptamer sequence binding to peptidoglycan-associated lipoprotein Legionella pneumophila, which is a sequence of single-chain DNA-aptamer AptPAL_23 with length of 81 nucleotides, with molecular weight of 28 kDa, with primary sequence CATACGTTCGACTGCTACGTGCATTCTTCTCCTGGGGGGGCTGCCGTCTCTACAGGGCATTATGTGAGATGTACAGACTAG, containing regions for binding with primers of length 18 nucleotides in terminal extreme positions, forming a three-dimensional structure forming stable specific bonds with PAL Legionella, having equilibrium dissociation constant (KD) with respect to PAL at least 5 × 10-8M.
EFFECT: invention enables detection of PAL legionella allergic antigen in test systems as a specific amplified matrix.
1 cl, 8 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PHARMACEUTICAL COMPOSITION FOR TREATMENT AND/OR PREVENTION OF MALIGNANT TUMOR | 2017 |
|
RU2766586C2 |
| PHARMACEUTICAL COMPOSITION FOR TREATING AND/OR PREVENTING MALIGNANT TUMOUR | 2016 |
|
RU2714205C2 |
| HERPES VACCINE | 2019 |
|
RU2731073C1 |
| RECOMBINANT FUSED PROTEIN | 2019 |
|
RU2732795C1 |
| GENETIC CONSTRUCT CODING HUMAN YB-1 PROTEIN PRECURSOR, ESCHERICHIA COLI STRAIN - PRODUCER OF HUMAN YB-1 PROTEIN PRECURSOR, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF SAID PRECURSOR | 2019 |
|
RU2728237C1 |
| MATERIALS AND METHODS USED FOR TREATMENT OF RESPIRATORY DISEASES IN DOGS | 2020 |
|
RU2811752C2 |
| MUCOADHESIVE MICROORGANISM | 2017 |
|
RU2762940C2 |
| ORAL CARE PRODUCTS AND METHODS CONTAINING HLP | 2016 |
|
RU2759134C2 |
| METHOD FOR MICROBIOLOGICAL SYNTHESIS OF BOVINE PROCHYMOSIN USING RECOMBINANT STRAIN PICHIA PASTORIS CONTAINING SYNTHETIC GENE OF PREPROCHYMOSIN VARIANT WITH MODIFIED SIGNALING SECRETION SEQUENCE | 2020 |
|
RU2779307C2 |
| METHOD FOR PRODUCING CHEMICAL PRODUCTS OF SUBTLE SYNTHESIS USING CORYNEBACTERIUM SECRETING MODIFIED a-1,6-GLUCOSIDASES | 2018 |
|
RU2763317C2 |
