FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and represents a DNA-aptamer sequence binding to peptidoglycan-associated lipoprotein Legionella pneumophila, which is a sequence of single-chain DNA-aptamer AptPAL_23 with length of 81 nucleotides, with molecular weight of 28 kDa, with primary sequence CATACGTTCGACTGCTACGTGCATTCTTCTCCTGGGGGGGCTGCCGTCTCTACAGGGCATTATGTGAGATGTACAGACTAG, containing regions for binding with primers of length 18 nucleotides in terminal extreme positions, forming a three-dimensional structure forming stable specific bonds with PAL Legionella, having equilibrium dissociation constant (KD) with respect to PAL at least 5 × 10-8M.
EFFECT: invention enables detection of PAL legionella allergic antigen in test systems as a specific amplified matrix.
1 cl, 8 dwg, 6 ex
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