METHOD OF PRODUCING POLYMERS FROM SPIDER SILK PROTEINS Russian patent published in 2015 - IPC C07K14/435 D01F4/00 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used to produce polymers from spider silk protein. The method includes obtaining spider silk protein from 240-760 amino acid residues of formula NT2-REP-CT or NT-REP-CT, where: NT is an N-terminal fragment of 100 to 160 amino acids, originating from spider silk protein, with at least 80% identity to SEQ ID NO: 6; REP is a protein fragment of 70 to 300 amino acids, selected from L(AG)_nL, L(AG)_nAL, L(GA)_nL, L(GA)_nGL, where: n is an integer from 2 to 10; A consists of 8-18 amino acids, where 0 to 3 amino acids are not Ala, and the rest of the amino acids are Ala; G consists of 12-30 amino acids, where at least 40% amino acids are Gly; and L is a linker consisting of 0-20 amino acids; CT is a C-terminal fragment of 70 to 120 amino acids, originating from spider silk protein, with at least 80% identity to SEQ ID NO: 7. The method includes dissolving the obtained spider silk protein in a liquid medium with pH 6.4 or higher and/or ionic strength higher than 300 mM to prevent polymerisation and increase solubility of spider silk protein with optional removal of lipopolysaccharides and other pyrogenes. Further, properties of said liquid medium are adjusted to pH 6.3 or lower and ionic strength lower than 300 mM to facilitate polymerisation. Spider silk protein polymers are separated from the liquid medium after polymerisation.

EFFECT: invention enables to control the assembling of spidroin polymers.

14 cl, 8 dwg, 3 tbl, 15 ex