POLYMERIZABLE RECOMBINANT SPIDROIN PROTEIN OF PRINCIPAL AMPULLARY GLAND, CONTROLLED POLYMERISABLE RECOMBINANT FUSED PROTEIN, SPIDROIN PROTEIN POLYMER OF PRINCIPAL AMPULLARY GLAND, CONTROLLED POLYMERISATION COMPOSITION, RECOMBINANT NUCLEIC ACID MOLECULE (VERSIONS), METHOD FOR PRODUCING SOLUBLE FUSED PROTEIN, METHOD FOR PRODUCING SPIDROIN PROTEIN POLYMER OF PRINCIPAL AMPULLARY GLAND AND METHOD FOR EUKARIOTIC CELL CULTURE Russian patent published in 2012 - IPC C07K14/435 C12N15/62 D01F4/02 

FIELD: medicine.

SUBSTANCE: recombinant method is used to produce a spidroin protein of a principal ampullary gland which consists of 150-420 amino acid residues and is described by formula REP-CT where REP is a protein fragment consisting of 80-300 amino acid residues, selected from L(AG)nL, L(AG)nAL, L(GA)nL or L(GA)nGL where n is an integer from 4 to 8. Each individual segment A consists of 8-18 amino acid residues, where 0 to 3 amino acid residues are not Ala, while the rest are Ala. Each individual segment G consists of 12-30 amino acid residues, where at least 40% of amino acid residues are Gly. Each individual segment L is a linker amino acid sequence of 0-20 amino acid residues. CN is a fragment consisting of 70 to 120 amino acid residues of a C-terminal spidroin protein fragment of the principal ampullary gland.

EFFECT: invention presents the polymerisable spidroin protein of the principal ampullary gland which is used as a basis to produce the controlled polymerisable fused protein.

15 cl, 7 dwg, 13 ex