FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology, namely to method of identifying presence of two or more proteins of interest with known amino acid sequences in sample of vegetable origin (versions) and method of preserving genotype of transgenic plant variety. Methods include cleavage of all proteins from sample of vegetable origin with sequence-specific protease with obtaining peptides. Peptides are separated in one step in device for liquid chromatography - mass spectrometry (LC-MS). Peptides are ionised in LC-MS device. Simultaneous obtaining of mass-spectral data for peptides in LC-MS device is performed. Said simultaneous mass-spectral data for peptides with expected mass-spectral data from proteins of interest are compared with known amino acid sequences. Determination of presence or absence of proteins of interest is realised.
EFFECT: claimed invention makes it possible to detect presence of two or more proteins of interest of vegetable origin with high efficiency.
17 cl, 10 dwg, 3 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRUCTURE AND METHOD OF CONSTRUCTION OF SYNTHETIC TWO-DIRECTIONAL VEGETABLE PROMOTER UBI1 | 2012 |
|
RU2639538C2 |
| METHOD AND STRUCTURE FOR SYNTHETIC TWO-DIRECTIONAL VEGETABLE PROMOTER SCBV | 2012 |
|
RU2627595C2 |
| STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1 RELATED TRANSGENIC SOYBEAN LINES AND DETECTION THEREOF | 2011 |
|
RU2608650C2 |
| METHOD OF CONTROLLING WEEDS USING PLANTS AAD-1 AND PRESOWING AND / OR PRE-EMERGENCE TREATMENT WITH HERBICIDES | 2012 |
|
RU2610664C2 |
| SYNTHETIC TRANSIT CHLOROPLAST PEPTIDES FROM BRASSICA | 2013 |
|
RU2636035C2 |
| COMBINATIONS INCLUDING PROTEINS Cry34Ab/35Ab AND Cry3Aa, FOR PREVENTING DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (Diabrotica spp.) | 2011 |
|
RU2582249C2 |
| FIGHTING COLEOPTEROUS PESTS | 2011 |
|
RU2599446C2 |
| SYNTHETIC TRANSIT CHLOROPLAST PEPTIDES DERIVED FROM BRASSICA | 2013 |
|
RU2626535C2 |
| COMBINATIONS WHICH INCLUDE PROTEINS Cry34Ab/35Ab AND Cry6Aa FOR PREVENTING DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (Diabrotica spp.) | 2011 |
|
RU2582262C2 |
| COMBINATIONS CONTAINING Cry34Ab/35Ab AND Sry3Ba PROTEINS, FOR PREVENTING DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (Diabrotica spp.) | 2011 |
|
RU2591519C2 |
