BILE RESISTANT BACILLUS COMPOSITION SECRETING HIGH LEVELS OF ESSENTIAL AMINO ACIDS Russian patent published in 2015 - IPC C12N1/20 A23K1/16 C12Q1/02 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to a method of screening and isolating Bacillus subtilis cells, an animal feed supplement composition containing said cells and an animal feeding method. The method includes collecting and isolating Bacillus subtilis spore cells, characterised by rapid germination and growth in the presence of a medium with 4 mM or 6 mM bile salts, obtaining vegetative cells from the spore cells, UV mutation of said vegetative cells, collecting and isolating vegetative Bacillus subtilis cells, characterised by a high level of producing at least one essential amino acid, analysing the obtained vegetative Bacillus subtilis cells to confirm maintenance of germination and growth in the presence of a medium with bile salts and isolating the collected Bacillus subtilis cells. The obtained cells are added in amount of 10⁵ to 10¹² CFU/g to the feed supplement composition. The method of feeding an animal includes administering said feed supplement composition to an animal along with other feed ingredients.

EFFECT: group of inventions has a probiotic effect on the body and increases digestion and availability of nutrients from animal feed.

8 cl, 3 dwg, 1 tbl, 3 ex