FIELD: biotechnology.
SUBSTANCE: group of inventions is described, including a method for purifying a subtype of Factor VIII (FVIII) (embodiments), where the subtype FVIII is defined as a single FVIII heavy chain that is associated with a single FVIII light chain. In one embodiment, the method includes the steps of: (1) anion exchange chromatography with a composition comprising several FVIII subtypes and collecting the eluate fraction comprising said FVIII subtypes to be purified, (2) size exclusion chromatography of the eluate fraction obtained in step (1) comprising said FVIII subtype and collecting the eluate comprising the specified purified FVIII subtypes; and (3) concentrating the fraction of the eluate obtained in step (2) comprising said FVIII subtypes to be purified.
FIELD: invention expands the range of methods for purification of the FVIII subtypes.
12 cl, 58 dwg, 29 tbl, 29 ex
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