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RU

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2 573 927

(13)

C2

(51)

IPC

A01H4/00(2006-01-01)

(21) (22)

Application

2013157526/10, 2011-06-07

(24)

Start date

2011-06-07

(22)

Actual filing date

2011-06-07

(45)

Published

2016-01-27

(72)

Inventor

Kirkhkhoff JaninaShillberg ShtefanShirmejer AndreasShinkel' Khel'GaFisher Rajner

(73)

Holder

Fraunkhofer-Gezell'Shaft Tsur Ferderung Der Angevandten Forshung E.F.

METHOD FOR OBTAINING MONOCLONAL LINE OF PLANT CELLS Russian patent published in 2016 - IPC A01H4/00 

Abstract RU 2573927 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Method includes obtaining heterologous population of plant cells and obtaining protoplasts from said heterologous population of plant cells. After that, separation of single protoplasts is realised by subjecting protoplast obtaining to flow-cytometric sorting. Then, regeneration of separated single protoplast is realised by joined cultivation in presence of material of feeder cells until microcolony is formed. Obtained microcolonies are separated from material of feeder cells and cultivated until monoclonal line of plant cells is formed.

EFFECT: claimed is method for obtaining monoclonal line of plant cells from heterologous population of plant cells, including the given stages.

5 cl, 1 dwg, 2 ex

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RU 2 573 927 C2

Authors

Kirkhkhoff Janina

Shillberg Shtefan

Shirmejer Andreas

Shinkel' Khel'Ga

Fisher Rajner

Dates

2016-01-27—Published

2011-06-07—Filed

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