FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, in particular to a method for integrating a nucleic acid sequence of interest in a FAD2 cell gene comprising site-specific cleavage of the FAD2 gene of a plant cell using a zinc finger nuclease. Site-specific zinc finger nuclease is also disclosed for use in modifying the FAD2 gene.
EFFECT: invention effectively integrates a nucleic acid sequence of interest into a plant cell.
7 cl, 24 dwg, 14 tbl, 10 ex
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