FIELD: biotechnology.
SUBSTANCE: invention relates to a set of synthetic oligonucleotides for species identification of Bupleurum multinerve (Bupleurum multinerve DC.), comprising polymerase chain reaction with a fragment ITS2 of nuclear DNA. And for identification species-specific portions are used to create the forward, reverse primers and destroyed probe, where the forward primer is the DNA sequence with the following nucleotide structure: 5'-TATCCCGGCTCTCGCATCGA-3'; the reverse primer is the DNA sequence with the following nucleotide structure: 5'-GACGAGGCACGGGAGGTCAG-3', the destroyed probe DNA sequence with the following nucleotide composition (fluorescent label)-5'-CCGTGAACCATCGAGTTTTT-3'-(quencher).
EFFECT: invention enables the species identification of Bupleurum multinerve during screening of plant raw materials.
1 dwg, 1 ex
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