FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for determining species identity of tissues of chickens and pigs in food raw materials, fodder and food products in a test system for determining species identity of tissues of chickens and pigs in food raw materials, fodder and food products, including plastic bottles and test tubes, a thermostable enzyme Tag-polymerase, a reaction buffer, a mixture of four deoxynucleotide triphosphates, specific DNA segments of several oligonucleotide primers, probes, an internal control sample in form of a bacteriophage suspension and positive control samples – containing fragments of DNA genomes of several types of meat, according to the invention, meat genome is represented by chicken tissues (Gallus gallus) and pig (Sus scrofa), wherein for internal control sample suspension of bacteriophage T4 with concentration of 5×103 copies of nucleotide sequences at 1 mcl, and for a positive control sample – a mixture containing fragments of genomes of chicken tissues ({Gallus gallus) and pig (Sus scrofa) and bacteriophage T4, taken in ratio 1:1:1.
EFFECT: invention allows to expand functional capabilities and increase accuracy of identification of species identity of pigs and hens tissues.
1 cl, 5 dwg, 5 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETERMINING SPECIES IDENTITY OF TISSUES OF CHICKENS AND PIGS IN FOOD RAW MATERIALS, FODDER AND FOOD PRODUCTS | 2018 |
|
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RU2726427C1 |
TEST SYSTEM FOR DETECTING DNA OF DONKEY (EQUUS ASINUS) TISSUE IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
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METHOD FOR WOODPECKER (PICIDAE) TISSUE DNA DETERMINATION IN DRY FEED AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
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TEST SYSTEM FOR IDENTIFICATION OF DNA OF DOMESTIC DOG TISSUE (CANIS LUPUS FAMILIARIS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
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Authors
Dates
2019-09-17—Published
2018-10-01—Filed