TEST SYSTEM FOR DETERMINING SPECIES AFFILIATION OF TISSUES OF CHICKENS AND PIGS IN FOOD RAW MATERIALS, FODDER AND FOOD PRODUCTS Russian patent published in 2019 - IPC C12Q1/68 

Abstract RU 2700480 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for determining species identity of tissues of chickens and pigs in food raw materials, fodder and food products in a test system for determining species identity of tissues of chickens and pigs in food raw materials, fodder and food products, including plastic bottles and test tubes, a thermostable enzyme Tag-polymerase, a reaction buffer, a mixture of four deoxynucleotide triphosphates, specific DNA segments of several oligonucleotide primers, probes, an internal control sample in form of a bacteriophage suspension and positive control samples – containing fragments of DNA genomes of several types of meat, according to the invention, meat genome is represented by chicken tissues (Gallus gallus) and pig (Sus scrofa), wherein for internal control sample suspension of bacteriophage T4 with concentration of 5×103 copies of nucleotide sequences at 1 mcl, and for a positive control sample – a mixture containing fragments of genomes of chicken tissues ({Gallus gallus) and pig (Sus scrofa) and bacteriophage T4, taken in ratio 1:1:1.

EFFECT: invention allows to expand functional capabilities and increase accuracy of identification of species identity of pigs and hens tissues.

1 cl, 5 dwg, 5 tbl

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RU 2 700 480 C1

Authors

Kotelnikova Aleksandra Andreevna

Chernykh Oleg Yurevich

Bannov Vasilij Aleksandrovich

Malyshev Denis Vladislavovich

Donnik Irina Mikhajlovna

Lysenko Aleksandr Anatolievich

Krivonos Roman Anatolevich

Shevkoplyas Vladimir Nikolaevich

Koshchaev Andrej Georgievich

Dajbova Lyubov Anatolevna

Fisinin Vladimir Ivanovich

Kochish Ivan Ivanovich

Stekolnikov Anatolij Aleksandrovich

Klimenko Aleksandr Ivanovich

Shakhov Aleksej Gavrilovich

Sukhanova Svetlana Failevna

Zabashta Nikolaj Nikolaevich

Drobin Yurij Dmitrievich

Dates

2019-09-17Published

2018-10-01Filed