FIELD: biotechnology.
SUBSTANCE: invention is a set of synthetic oligonucleotides for species identification of roseroot (Rhodiola quadrifida (Pall.) Fisch. et Mey). The set comprises species-specific areas to create a forward, reverse primers and a destructible probe, namely forward primer - CGGCAACGGATATCTCGGCT-3', the reverse primer - 5'-GGCCTCGCAACCACCACTTGTC-3', the destructible probe - (fluorescent label)-5'-CCGTGAACCATCGAGTTTTT-3'-(quencher).
EFFECT: invention enables to identify reliably, fast and with high sensitivity the species of medicinal plant in the course of screening of plant raw material.
1 dwg, 1 ex
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Authors
Dates
2014-08-20—Published
2013-06-10—Filed