FIELD: bioengineering.
SUBSTANCE: method of the molecular-gene intragroup typing of Vibrio cholerae O1 and O139 serogroups. The method includes the DNA extraction from the studied strain V. cholerae of serogroup O1 or O139, PCR setting with specific primers and accounting of the reaction by electrophoresis. The amplification of the studied DNA is performed by designed specific primers for each of six INDEL-genes, namely for genes ompU, 1606, eamA, 096, CoA and cheA, each of them having two discriminate alleles. The accounting of the typing results is performed as visual identification after electrophoresis, wherein for each studied strain the unique INDEL-gene type is determined by two or more of the INDEL-genes. By the comparison of the determined INDEL-gene types the total or different origin of the studied strains V.cholerae O1 and O139 serogroups is determined.
EFFECT: invention determines unique gene markers of the said strains, this can be used in the work of special institutions involved in cholera monitoring.
1 dwg, 4 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIFFERENTIATING TOXIGENIC AND ANTITOXIGENIC CHOLERA VIBRIOS STRAINS SEROGROUP O1 BY INHIBITING ACTIVITY | 2015 |
|
RU2596401C1 |
| METHOD FOR ASSESSING INTRASPECIFIC COMPETITION OF TOXIGENIC AND NON-TOXIGENIC STRAINS OF VIBRIO CHOLERAE O1 AND O139 SEROGROUPS USING REAL-TIME PCR | 2023 |
|
RU2806564C1 |
| METHOD FOR MODELING BIOFILMS FORMED BY VIBRIO CHOLERAE 01 SEROGROUP ON CHITIN SURFACE | 2018 |
|
RU2685878C1 |
| METHOD FOR MOLECULAR TYPING FOR TYPICAL (O1) AND ATYPICAL (RO) NON-TOXIGENIC V. CHOLERAE EL TOR STRAINS USING REAL-TIME PCR | 2022 |
|
RU2792156C1 |
| METHOD FOR DETERMINATION OF GENETIC RELATIONSHIP OF COMMA BACILLI STRAINS BY SEQUENCING GENES FLANKING CLUSTER OF O-ANTIGEN BIOSYNTHESIS GENES | 2008 |
|
RU2393231C1 |
| METHOD FOR MOLECULAR GENETIC TYPING OF KLEBSIELLA PNEUMONIAE STRAINS USING INDEL MARKERS | 2022 |
|
RU2796431C1 |
| METHOD FOR IDENTIFICATION OF TOXIGENIC GENETIC VARIANTS OF CHOLERA CAUSATIVE AGENT EL TOR WITH SET OF MUTATIONS IN GENES OF VIRULENCE AND PANDEMIC ISLAND GENES BY MULTIPLEX POLYMERASE CHAIN REACTION | 2022 |
|
RU2815711C2 |
| METHOD OF SIMULTANEOUS IDENTIFICATION OF TOXIGENIC STRAINS OF GENOVARIANTS OF EL TOR CHOLERA CAUSATIVE AGENT AND THEIR DIFFERENTIATION ON EPIDEMIC POTENTIAL BY METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2560280C2 |
| METHOD OF IDENTIFICATION OF NON-OXYGENIC STAMPS OF CHILLER VIBRIONS OF O1 SEROGRUPP BY PCR FOR OBTAINING THE GENETIC DETERMINANTS | 2017 |
|
RU2665542C1 |
| METHOD FOR IDENTIFYING TOXIGENIC V CHOLERAE O1 STRAINS, DETERMINING ITS BIOVAR AND DIFFERENTIATING BIOVAR ELTOR STRAINS BY TYPICAL AND CHANGED BY MULTIPLEX POLYMERASE CHAIN REACTION, AND TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2011 |
|
RU2458141C1 |
